SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Analysis of the intronic single nucleotide polymorphism rs#466452 of the nephrin gene in patients with diabetic nephropathy

Du,Guankui; Xiao,Man; Zhang,Xuezi; Wen,Maoyu; Pang,Chi; Jiang,Shangfei; Sang,Shenggang; Xie,Yiqiang.

Abstract Background A number of dysregulated miRNAs have been identified and are proposed to have significant roles in the pathogenesis of type 2 diabetes mellitus or renal pathology. Alpinia oxyphylla has shown significant anti-inflammatory properties and play an anti-diabetes role. The objective of this study was to detect the alteration of miRNAs underlying the anti-diabetes effects of A. oxyphylla extract (AOE) in a type II diabetic animal model (C57BIKsj db-/db-). Results Treatment with AOE for 8 weeks led to lower concentrations of blood glucose, urine albumin, and urine creatinine. 17 and 13 miRNAs were statistically identified as differentially regulated in the DB/DB and db-/db- AOE mice, respectively, compared to the untreated db-/db- mice. Of...

Tipo: Journal article

Palavras-chave: MiRNA; Db-/db- mice; Alpinia oxyphylla Miq; Kidney; Diabetic nephropathy.

Ano: 2017

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100206

Comparison of methods for urinary albumin determination in patients with type 1 diabetes

GONZÁLEZ,RODRIGO; TIRADO,ARES; ROJAS,LUIS A; OSSANDÓN,FRANCISCO J; ALVO,MIRIAM; WOLFF,CARLOS; SEELENFREUND,DANIELA; DURRUTY,PILAR; LOBOS,SERGIO.

We present the analysis of an intronic polymorphism of the nephrin gene and its relationship to the development of diabetic nephropathy in a study of diabetes type 1 and type 2 patients. The frequency of the single nucleotide polymorphism rs#466452 in the nephrin gene was determined in 231 patients and control subjects. The C/T status of the polymorphism was assessed using restriction enzyme digestions and the nephrin transcript from a kidney biopsy was examined. Association between the polymorphism and clinical parameters was evaluated using multivaríate correspondence analysis. A bioinformatics analysis of the single nucleotide polymorphism rs#466452 suggested the appearance of a splicing enhancer sequence in intron 24 of the nephrin gene and a...

Tipo: Journal article

Palavras-chave: Diabetic nephropathy; NPHS1; Polymorphism; Splicing enhancer.

Ano: 2009

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200007

Khawali,C.; Andriolo,A.; Ferreira,S.R.G..

We tested the correlation of the albumin-to-creatinine ratio (A/C) in an early-morning urine sample, measured with a commercial kit (DCA 2000®), with the conventional immunoturbidimetric determination in the laboratory and with overnight albumin excretion rate (reference method). Fifty-five type 1 diabetic adolescents had their first-morning urine collected on the 1st and 8th day of the period. Urinary albumin and creatinine were determined immediately using the DCA 2000® kit. Samples were also stored for laboratory analysis. To evaluate the correlation between early-morning urinary A/C ratio and overnight albumin excretion rate, 16 subjects had a timed overnight urine collection. A/C ratios determined with the DCA 2000® kit and by the laboratory method...

Epigallocatechin-3-gallate combined with alpha lipoic acid attenuates high glucose-induced receptor for advanced glycation end products (RAGE) expression in human embryonic kidney cells

Palavras-chave: Diabetic nephropathy; Microalbuminuria; Screening.

Ano: 2002

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000300008

Anais da ABC (AABC)

Leu,Jyh-Gang; Lin,Chin-Yao; Jian,Jhin-Hao; Shih,Chin-Yu; Liang,Yao-Jen.

The anti-oxidant effects of epigallocatechin gallate (EGCG) and alpha lipoic acid (ALA) have been demonstrated in previous studies. The kidney protection effects of EGCG and ALA in patients with kidney injury are still under investigation. The purpose of this study is to investigate the anti-inflammatory and anti-oxidant effects of EGCG and ALA on high glucose-induced human kidney cell damage. EGCG inhibited high glucose(HG)-induced TNF-α and IL-6 production in human embryonic kidney (HEK) cells. Both EGCG and ALA decreased HG-induced receptor of advanced glycation end products (RAGE) mRNA and protein expressions in HEK cells. EGCG and ALA also recovered HG-inhibited superoxide dismutase production and decreased ROS expressions in HEK cells. The synergism...

Factors associated with the development of renal complications of diabetes mellitus in São Paulo city

Palavras-chave: Diabetic nephropathy; Receptor of advanced glycation end products; Epigallocatechin gallate; Alpha lipoic acid; Anti-oxidant.

Ano: 2013

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000200745

lncRNA ZEB1-AS1 inhibits high glucose-induced EMT and fibrogenesis by regulating the miR-216a-5p/BMP7 axis in diabetic nephropathy

The incidence of diabetic end-stage renal failure (ESRF) varies worldwide and risk factors have been demonstrated in several populations. The objective of the present study was to identify possible factors associated with the risk of development of ESRF in patients with diabetes mellitus (DM). Two groups of diabetic subjects were included in a case-control study: 1) one group was submitted to renal replacement therapies, attending dialysis centers in São Paulo city and 2) the same number of controls without clinical nephropathy (two negative dipstick tests for urine protein), matched for duration of DM, were obtained from an outpatient clinic. A standardized questionnaire was used by a single investigator and additional data were obtained from the medical...

Tipo: Info:eu-repo/semantics/other

Palavras-chave: Chronic renal failure; Diabetes mellitus; Diabetic nephropathy; Associated factors.

Ano: 1997

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000600006

Meng,Qingqing; Zhai,Xiaolin; Yuan,Yi; Ji,Qing; Zhang,Pengyuan.

Diabetic nephropathy (DN) is one of the leading causes of mortality in diabetic patients. Long non-coding RNA zinc finger E-box binding homeobox 1 antisense 1 (ZEB1-AS1) plays a crucial role in the development of various diseases, including DN. However, the molecular mechanism of ZEB1-AS1 in DN pathogenesis remains elusive. An in vitro DN model was established by treating HK-2 cells with high glucose (HG). Quantitative polymerase chain reaction (qRT-PCR) was utilized to detect the expression levels of ZEB1-AS1, microRNA-216a-5p (miR-216a-5p), and bone morphogenetic protein 7 (BMP7). Western blot assay was used to evaluate the protein levels of BMP7, epithelial-to-mesenchymal transition (EMT)-related proteins, and fibrosis markers. Additionally, the...

MSC transplantation: a promising therapeutic strategy to manage the onset and progression of diabetic nephropathy

Palavras-chave: ZEB1-AS1; MiR-216a-5p; BMP7; Diabetic nephropathy; EMT; Fibrogenesis.

Ano: 2020

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000400607

Ezquer,Marcelo E; Ezquer,Fernando E; Arango-Rodríguez,Martha L; Conget,Paulette A.

Currently, one of the main threats to public health is diabetes mellitus. Its most detrimental complication is diabetic nephropathy (DN), a clinical syndrome associated with kidney damage and an increased risk of cardiovascular disease. Irrespective of the type of diabetes, DN follows a well-known temporal course. The earliest detectable signs are microalbuminuria and histopathological changes including extracellular matrix deposition, glomerular basement membrane thickening, glomerular and mesangial expansion. Later on macroalbuminuria appears, followed by a progressive decline in glomerular filtration rate and the loss of glomerular podocytes, tubulointerstitial fibrosis, glomerulosclerosis and arteriolar hyalinosis. Tight glycemic and hypertension...

Tipo: Journal article

Palavras-chave: Regenerative medicine; Diabetes mellitus; Diabetic nephropathy; Multipotent mesenchymal stromal cells; Mesenchymal stem cells.

Ano: 2012

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000300010

New Insights into the Roles of Megalin/LRP2 and the Regulation of its Functional Expression

Marzolo,María-Paz; Farfán,Pamela.

Since the discovery of the low-density lipoprotein receptor (LDLR) and its association with familial hypercholesterolemia in the early 1980s, a family of structurally related proteins has been discovered that has apolipoprotein E as a common ligand, and the broad functions of its members have been described. LRP2, or megalin, is a member of the LDLR family and was initially called gp330. Megalin is an endocytic receptor expressed on the apical surface of several epithelial cells that internalizes a variety of ligands including nutrients, hormones and their carrier proteins, signaling molecules, morphogens, and extracellular matrix proteins. Once internalized, these ligands are directed to the lysosomal degradation pathway or transported by transcytosis...

Tipo: Journal article

Palavras-chave: Alzheimer's Disease; Cubilin; Diabetic nephropathy; FXR; Gallstone disease; GSK3; Lowe syndrome; Megalin; PPAR; RAP; Regeneration.

Ano: 2011

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000100012

Proliferative diabetic retinopathy is associated with microalbuminuria in patients with type 2 diabetes

Boelter,M.C.; Gross,J.L.; Canani,L.H.; Costa,L.A.; Lisboa,H.R.; Três,G.S.; Lavinsky,J.; Azevedo,M.J..

Diabetic retinopathy is one of the leading causes of blindness in working-age individuals. Diabetic patients with proteinuria or those on dialysis usually present severe forms of diabetic retinopathy, but the association of diabetic retinopathy with early stages of diabetic nephropathy has not been entirely established. A cross-sectional study was conducted on 1214 type 2 diabetic patients to determine whether microalbuminuria is associated with proliferative diabetic retinopathy in these patients. Patients were evaluated by direct and indirect ophthalmoscopy and grouped according to the presence or absence of proliferative diabetic retinopathy. The agreement of diabetic retinopathy classification performed by ophthalmoscopy and by stereoscopic color...

Reduced cortical renal GLUT1 expression induced by angiotensin-converting enzyme inhibition in diabetic spontaneously hypertensive rats

Palavras-chave: Diabetic retinopathy; Diabetic nephropathy; Microalbuminuria; Type 2 diabetes mellitus.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000800006

Souza,M.S.; Machado,U.F.; Okamoto,M.; Bertoluci,M.C.; Ponpermeyer,C.; Leguisamo,N.; Azambuja,F.; Irigoyen,M.C.; Schaan,B.D..

Diabetes in spontaneously hypertensive rats is associated with cortical renal GLUT1 and GLUT2 overexpression. Our objective was to evaluate the effect of the angiotensin-converting enzyme blockade on cortical renal GLUT1 and GLUT2 expression, urinary albumin and urinary TGF-β1. Streptozotocin, 50 mg/kg, or citrate buffer (N = 16) was administered as a single injection into the tail vein in adult spontaneously hypertensive rats (~260 g). Thirty days later, these diabetic spontaneously hypertensive rats received ramipril by gavage: 0.01 mg·kg-1·day-1 (D0.01, N = 14), 1 mg·kg-1·day-1 (D1, N = 9) or water (D, N = 11) for 15 days. Albumin and TGF-β1 (24-h urine), direct arterial pressure, renal tissue angiotensin-converting enzyme activity (fluorometric assay),...

Transforming growth factor beta activity in urine of patients with type 2 diabetes and diabetic nephropathy

Palavras-chave: Angiotensin-converting enzyme inhibition; Diabetes mellitus; Diabetic nephropathy; Glucose transporter proteins; Hypertension; Streptozotocin.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001100004